Notch Signaling in Acquired Middle Ear Cholesteatoma

Hypothesis: We hypothesized that an anomalous change of Notch signaling might be involved in the pathophysiology cholesteatoma. Background: The pathway regulates integrated growth and differentiation control keratinocytes. Its involvement cholesteatoma proliferation has not been elucidated. Methods: obtained external auditory canal (EAC) skin samples from patients with middle ear who underwent tympanomastoid surgery. performed polymerase chain reaction using RT2 Profiler™ PCR Array Human Signaling Pathway (Qiagen) EAC (n = 6 each). This was followed by immunohistochemical staining Notch1, enhancer split-1 (HES1), p53 41 8 samples, respectively. Results: fold Notch1 gene expression lowest cholesteatoma, a statistically significant difference (p 0.0424). Moreover, HES1 decreased 0.272). positive rates protein expressions (48.5 ± 32.4% 44.9 17.8%, respectively) were significantly lower than (83.4 17.5% 55.7 7.1%, < 0.001 p 0.01). In contrast, rate (8.5 11.4%) higher (0.5 0.7%) 0.001). Conclusion: decreases play important role hyperproliferative character keratinizing squamous epithelium An increase reflect to cellular hyperproliferation.